作　　者： ; ; ;

机构地区： 东北农业大学食品学院

出　　处： 《中国粮油学报》 2009年第11期33-37,共5页

摘　　要： 研究了预处理条件对大豆分离蛋白水解效果的影响,热处理与微波辅助处理相结合能显著提高蛋白酶的水解效率。比较了6种酶解大豆蛋白产物的ACE抑制活性,选择碱性蛋白酶为最佳水解用酶,并优化了酶解条件。研究了8种大孔树脂对水解产物精制效果,结果表明：经D3520型大孔吸附树脂精制后,水解产物脱盐率89.4%,肽回收率90.6%,ACE抑制活性达84.1%。经HPLC法测定,大豆分离蛋白ACE活性抑制肽混合物分子质量主要分布在200-800 ku之间。 The pretreatment conditions of soy protein isolate（SPI） hydrolysis were tested,and alcalase was selected from six enzymes for the SPI hydrolysis to prepare antihypertensive peptides with high inhibitory activity for angiotensin converting enzyme（ACE）.Orthogonal design of four factors three levels was taken to optimize the hydrolysis conditions of SPI with alcalase protease.Finally,eight kinds of macroporous adsorbing resins were compared for purification of the produced antihypertensive peptides,and D3520 was selected.Results： After the purification,the recovery rate of antihypertensive peptides is more than 90.6%,and the ACE inhibitory activity of the product is 84.1%.The result of HPLC shows that the relative molecular weights of the antihypertensive peptides mainly distribute from 200 to 800 ku.

关 键 词： 大豆分离蛋白 血管紧张素转换酶 活性抑制肽 酶解

领　　域： [轻工技术与工程] [轻工技术与工程]