作　　者： ; ;

机构地区： 华南理工大学轻工与食品学院

出　　处： 《现代食品科技》 2009年第11期1320-1322,1319,共4页

摘　　要： 应用南极假丝酵母脂肪酶B(Novozym435)在非水相体系(叔丁醇)催化乳酸和乙醇合成乳酸乙酯,通过单因素实验考察了温度、摇床转速、酶添加量、乳酸浓度、酸醇摩尔比(乳酸:乙醇)等条件对乳酸乙酯产率的影响,进一步应用响应曲面法优化了乳酸浓度、酸醇摩尔比、酶浓度等因素,获得乳酸乙酯合成的最佳条件:乳酸含量0.42mol/L、酸醇摩尔比1:6、酶浓度42g/mol、温度为50℃、摇床转速为200r/min,在此条件下乳酸乙酯的实际产率为70.21%±0.2%。 Immobilized Candida antarctica lipase B （Novozym 435）, was employed as catalyst for the synthesis of ethyl lactate using lactic acid and ethanol as substrates in tert-butyl alcohol. Based on single factor experiment, effects of reaction conditions on the yield of ethyl lactate were discussed, including temperature, shaking speed, the molar ratio of lactic acid to alcohol and dosages of enzyme and lactic acid. Furthermore, the concentration of lactic acid, the concentration of lipase and the molar ratio of acid to alcohol were optimized by response surface methodology （RSM）. The highest yield of ethyl lactate （70.21%±0.2%） was obtained under the following conditions： concentration of lactic acid being 0.42 mol/L, the molar ratio of acid to alcohol being 1：6, the dosage of lipase being 42 g/mol, the temperature being 50 ℃, and the shaking speed being 200 r/min.

关 键 词： 乳酸乙酯 脂肪酶 非水相 酯化反应

领　　域： [轻工技术与工程] [轻工技术与工程]