作　　者： ; ; ; ; ; ; ; ;

机构地区： 江西农业大学动物科学技术学院动物生物技术国家重点实验室培育基地

出　　处： 《畜牧兽医学报》 2009年第10期1435-1439,共5页

摘　　要： 为研究影响猪后腿质量的QTL,采用分布于猪全基因组19对染色体上的183个微卫星标记,对白色杜洛克(♂)×二花脸(♀)资源家系群体1 028头F2代个体及其亲本进行基因型检测,利用最小二乘线性回归分析,通过置换试验确定显著性域值,对影响猪后腿质量的数量性状位点(QTL)进行了定位分析。共检测到10个QTL位点,其中在猪2、4、7、8、18号染色体上检测到5个1%基因组显著水平的QTLs,在5和7号染色体上检测到2个5%基因组显著水平QTLs。影响效应最大的QTL位于7号染色体58 cM处,置信区间仅为5 cM,这些结果为下一步的精细定位和位置候选基因的分离奠定了基础。 To detect the QTL for ham weight （HW）, a total of 1 028 F2 animals at 240 days of age from a White Duroc× Erhualian resource population were measured for phenotypic data. All F2 individuals and their parents and grandparents were genotyped for 183 informative mierosatellite markers on 19 pairs of porcine chromosomes. Quantitative trait loci （QTL） for ham weight were identified by using an interval mapping method based on least-squares regression. The threshold values of significance were determined empirically by a permutation test. In total, 10 QTLs were detected including five 1% genome-wide significant QTLs on pig chromosomes （SSC） 2, 4, 7, 8 and 18 and two QTLs at the 5% genome-wide significant level on SSC5 and SSC7. The most significant QTL was found at 58 cM on SSC7 with a confidence interval of 5 cM. The results provide a start point to refine the QTLs for the interest traits and isolate positional candidate genes.

关 键 词： 猪 后腿质量 数量性状位点

领　　域： [农业科学] [农业科学] [生物学]