作　　者： ; ; ; ;

机构地区： 石河子大学农学院新疆生产建设兵团绿洲生态农业重点实验室

出　　处： 《中国农业科技导报》 2009年第4期53-57,共5页

摘　　要： microRNAs（miRNAs）是真核生物中一类长度约为22 nt的非编码小分子RNA,miRNA与AGO等蛋白形成RISC沉默复合体,通过剪切或翻译抑制对靶基因起负调控作用。对拟南芥miRNA序列及其配对的靶序列间的特征进行了统计分析,结果表明miRNA序列5′端富含A、U,第1、第19碱基位对U、C具有较强的倾向性;miRNA与靶序列间常有1～4个碱基错配,错配碱基常出现在第1,第2和第21位,而第3～第6,第9～第10,第16～第17碱基配对较为保守,为人工合成miRNA的设计及miRNA靶基因的预测以提供了依据。 MieroRNAs （miRNAs） are a class of endogenous non-coding single-stranded RNA with about 22 nucleotides length. MicroRNAs function as sequence-specific negative regulators in post-transcriptional gene silencing by base pairing with target mRNAs, which lead to mRNA cleavage or translational repression. We examined the sequence characteristics of all known Arabidopsis miRNAs and the base-pairing feature between miRNAs and their targets. The miRNA sequences displayed higher A and U content at the 5＇ end, and higher U content at position 1, and higher C content around position 19. There often exist one to four mismatching between miRNAs and their targets, which showed that mismatches occurred frequently at position 1,2 or 21, hut hardly occurred at position 3- 6, 9 - 10, 16 - 17. The data is potentially applicable to the design of artificial microRNA and prediction of microRNAs targets.

关 键 词： 拟南芥 基因沉默 微小 人工小

领　　域： [生物学]