作　　者： ; ; ; ; ;

机构地区： 天津科技大学生物工程学院工业微生物教育部重点实验室

出　　处： 《食品研究与开发》 2009年第11期148-151,共4页

摘　　要： 通过对茯苓药材中提取的茯苓多糖进行羧甲基修饰,制备一种水溶性羧甲基茯苓多糖(CMP),并对其抗氧化性进行研究。测定样品的还原能力和抗氧化能力两个指标:利用H2O2和Fe2+混合发生Fenton反应测定CMP对·OH的清除能力,采用邻苯三酚自氧化法测定CMP对·O2-的清除能力。结果显示在质量浓度为0.1g/L～5g/L的有效范围内该多糖具有一定的还原能力。在有效范围内CMP对·OH的清除50%羟自由基的多糖浓度(EC50)为2.5g/L,CMP浓度为5g/L时对·O2-的清除率为17%。 A water-soluble carboxymethylated Poria cocos polysaccharide （CMP） was prepared by the reaction with monochloroacetic acid. The antioxidative activities of CMP were also studied. Determine scavenging capacity of CMP to · OH by Fenton Reaction caused by mixing H2O2 and FeE ＋. And determine scavenging capacity of CMP to · O2^- by means of pyrogallol self- oxidation. The CMP had proper reducing capacity at the range of 0.1 g/L-5 g/L, EC50 of scavenging capability to ·OH was 2.5 g/L within the valid range, and the scavenging capacity of CMP to ·O2^- was 17 % with 5 g/L.

关 键 词： 茯苓多糖 羧甲基化 抗氧化

领　　域： [轻工技术与工程] [轻工技术与工程] [理学] [理学]