作　　者： ; ; ;

机构地区： 安徽理工大学医学院

出　　处： 《生物学杂志》 2009年第5期4-6,共3页

摘　　要： HCMV是一种广泛存在的疱疹病毒,在免疫抑制和免疫功能低下人群中,HCMV感染可引起严重疾病。RNase P是细胞内催化tRNA5′末端成熟的酶,当EGSs与靶mRNA互补结合并形成类似tNRA的复合物时,大肠杆菌RNase P催化亚基M1 RNA可具备对靶mRNA特异的催化切割活性。为研究抗病毒制剂,针对HCMV DNA多聚酶UL54 mRNA设计并构建特异性的EGS-C6,通过对UL54基因亚克隆片段转录产物体外切割研究,证实该EGS具备引导M1 RNA对UL54 mRNA特异切割的能力,可发展成一种新型抗病毒制剂。 HCMV is a ubiquitous herpesvirus. Infections by this virus can cause severe health problems in immunosuppressed and immunoeompromised individuals. RNase P is an enzyme which catalyzes a hydrolysis reaction to remove the leader sequence of precursor tRNA. When external guide sequences hybridize to the mRNA to form a structure resembling a tRNA substrate, M1 RNA from Escherichia coli can cleave the target mRNA specifically. To research antiviral agents, a sequence-specific EGS-C6 was used to target UL54 mRNA encoding major DNA polymerase of human cytomegalovirus. The results showed that EGS-C6 could direct the specific cleavage of UL54 mRNA in vitro and could be developed as a novel antiviral agent.

关 键 词： 外部引导序列 人巨细胞病毒 基因

领　　域： [生物学]