作　　者： ; ; ; ; ;

机构地区： 宁波大学信息科学与工程学院宁波市新型功能材料及其制备科学国家重点实验室培育基地

出　　处： 《分析化学》 2009年第8期1125-1130,共6页

摘　　要： 在玻碳电极(GCE)表面固定对H2O2有催化还原活性的富马酸二甲酯联吡啶铜(GCE|CuL);再在GCE|CuL表面修饰一层金磁微粒-壳聚糖复合膜(nanoAu/Fe3O4/Chit),进而固定艾滋病毒(HIV)诊断标志物——包膜糖蛋白(gp160)抗体(antigp160),由此构建了一类快速检测gp160的无试剂安培免疫传感器。当该传感器在含gp160溶液中37℃下温育30min后,传感器表面生成的免疫复合物随gp160浓度的增大而增加,导致CuL对H2O2电催化还原效果降低,催化电流呈现下降趋势。在PBS溶液(pH7.0)和-300mV下,催化电流的降低值ΔIo与gp160浓度在1～400μg/L呈线性关系;检出限为0.5μg/L(3σ)。研制的免疫传感器检测gp160时,一步免疫反应即可得结果,较相同条件下包被gp160抗体的纳米金单分子层修饰电极灵敏度更高,检测范围更宽,有望用于艾滋病人血清标志物gp160快速筛测。 A novel amperometric immunosensor for human immunodefficiency virus （HIV） glyeoprotein 160 （gp160） based on nanoAu/Fe3O4/Chitosan（chit） composite film as immobilization matrix was investigated. Firstly, the complex [ Cu （bpy） 2 （H2O） 12 （ C6H8O4 ） 2 （ CIO4 ） 4 （CuL） was prepared and adsorbed on the surface of glassy carbon electrode （GCE）, then nanoAu/Fe3O4/Chitosan composite film was modified on the surface of GCEI CuL and HIV gp160 antibody was furthermore immobilized on the surface to form one gp160 immunosensor（GCEICuL/nanoAu/Fe3O4/Chit/anti gp160）. CuL can be employed as an electron transfer mediator for catalytic activity detection of H2O2. After the immunosensor was incubated with HIV gp160 solution at 37℃ for 30 mix, the electron transfer access of CuL to H2O2 is partly inhibited, which led to a linear decrease of the catalytic efficiency to H2O2 by the oxidation of immobilized CuL by H2O2 at -300 mV in pH 7.0 PBS. Under the optimal conditions, the linear range of gp160 by the immunosensor is 1 -400μg/L and the detection limit is 0. 5 μg/L at 3 times noise. The immunosensor shows more sensitivity and accuracy than that only modified by gp160 antibody coated nano Au/chitosan film. This method simplifies the detection system without adding electron transfer media to solution and shortens the analytical time with one-step immunoassay, thus would be valuable for clinical immunoassay for HIV gp160 in AIDs patients＇ serums

关 键 词： 富马酸二甲酯联吡啶铜 纳米金 磁性微粒 壳聚糖 艾滋病毒 包膜糖蛋白 安培免疫传感器

领　　域： [理学] [理学] [自动化与计算机技术] [自动化与计算机技术]