作　　者： ; ; ; ; ;

机构地区： 南京农业大学动物科技学院

出　　处： 《南京农业大学学报》 2009年第4期148-153,共6页

摘　　要： 为研究纤维素酶与木聚糖酶对象草(Pennisetum purpureum Schumach.)青贮品质的影响,本试验共设6组:T1:象草+500 mg.kg-1纤维素酶;T2:象草+250 mg.kg-1纤维素酶;T3:象草+250 mg.kg-1木聚糖酶+250 mg.kg-1纤维素酶;T4:象草+500 mg.kg-1木聚糖酶;T5:象草+250 mg.kg-1木聚糖酶;C(对照组):象草。切短的象草经酶处理后在25℃条件下青贮在发酵瓶内,分别在青贮第2、4、6、8、15和30天从每组取出3瓶青贮样品用于发酵品质测定。结果表明:与对照组相比,纤维素酶与木聚糖酶或两者复合处理均能极显著降低青贮料的pH值(P<0.01),增加水溶性碳水化合物(WSC)含量(P<0.01)和乳酸(LA)含量(P<0.01)。青贮30 d结束时,除T3组外,其他酶处理组NH3-N含量显著低于对照组(P<0.05);各组中乙酸含量差异均不显著(P>0.05)。结论:添加纤维素酶或木聚糖酶均能改善象草青贮品质,并以纤维素酶与木聚糖酶复合处理效果最好。 This experiment was carried out to investigate the effects of cellulase and xylanase on fermentation quality of elephant grass （Pennisetum purpureum Schumach. ） silages during ensiling. Chopped elephant grass were ensiled in laboratory mini-silos either untreated （control） or treated with cellulase （500 or 250 mg·kg^-1 ）, cellulase （250 mg·kg^-1 ） plus xylanase （250 mg·kg^-1 ） , xylanase （500 or 250 mg·kg^-l ） , respectively. Triplicate silos for each treatment were opened at 2, 4, 6, 8, 15 and 30 days of ensifing with room temperature and analyzed for chemical characteristics. The enzyme treatments enhanced significantly production of lactic acid （P〈0. 01 ） and water soluble carbohydrate （P〈0. 01 ） of silages, and reduced rapidly the pH value （P〈 0. 01 ） compared with control. The contents of ammonia nitrogen in the enzymes treated silages, except for cellulase plus xylanase treated, was lower than in the untreated silage （P〈0. 05）. The contents of acetic acid were of no difference in all silages （P〉 0. 05）. The results suggested that the cellulase and xylanase improved the fermentation quality of elephant grass silage during ensiling.

关 键 词： 纤维素酶 木聚糖酶 象草 青贮

领　　域： [农业科学]