作　　者： ; ; ; ; ;

机构地区： 暨南大学生命科学技术学院

出　　处： 《生态毒理学报》 2009年第4期537-543,共7页

摘　　要： 鳜鱼(Siniperca chuatsi)是我国著名的肉食性鱼类,容易成为环境毒素的富集体.论文从分子水平上分析了鳜鱼去毒过程中起关键作用的谷胱甘肽S-转移酶(GST)基因的结构及进化上的地位,采用RT-PCR及RACE法,分离、克隆得到了鳜鱼肝脏可溶性alpha型(GSTA)和rho型(GSTR)基因cDNA全序列.结果表明,鳜鱼肝脏GSTA、GSTR全长分别为1052bp、935bp,其中5′-UTR分别为118bp、55bp,3′-UTR分别为262bp、202bp,分别编码223、225个氨基酸.系统分析结果显示:鳜鱼GSTA与GSTR在进化树上的位置与其分类所处的位置基本吻合. As an acanthomorph fish, Chinese perch （Siniperca chuatsi）was easily contaminated by toxin. This paper analyzed the structure and evolution position of soluble glutathione S -transferase （GST）in Chinese perch, which played an important role in detoxification. We got the alpha and rho GST （GSTA and GSTR）cDNA sequences from the liver of Chinese perch by RT -PCR and RACE. Results showed that total length of GSTA and GSTR were 1052bp and 935bp, respectively, flanked by 118bp 5′ UTR, 262bp 3′ UTR and 55bp 5′ UTR, 202bp 3′ UTR, respectively, and encoding 223 and 225 amino acid, respectively. In addition, the position of GSTA and GSTR in phylogenetic trees was similar to its classification position.

关 键 词： 谷胱甘肽 转移酶 基因克隆 序列分析 鳜鱼

领　　域： [生物学]