作　　者： ; ; ;

机构地区： 广州大学生命科学学院

出　　处： 《广东化工》 2009年第10期11-13,共3页

摘　　要： 以褐黄孢链霉菌GX-33为出发菌株,利用N+注入诱变,琼脂块初筛,结合了摇瓶复筛,获得了一株摇瓶效价能够稳定在3597～3625μg/mL水平的纳他霉素生产菌株,为后续生产提供了较优良的生产菌种。N+离子注入诱变纳他霉素产生菌在5×1015～1×1016N+/cm2剂量这一区域内,存活率比较高,为理想的诱变剂量区域。 A natamycine high yielding strain, based on, were obtaind by the technology of ion implantation followed by primary screening in agar block and second screening in shaking flasks. Natamycine yield of this strain maintained at the level of3597-3625/ag/mL, the results indicated that the obtained strain could be used as a promising strain for natamycine production. The ideal dosage of N＋ ion implantation for the survial rate of Streptomyces.gilvosporeus GX-33 was 5 × 10^15-1 × 10^16N＋/cm2.

关 键 词： 纳他霉素 褐黄孢链霉菌 离子 诱变育种

领　　域： [生物学]