作　　者： ; ; ; ;

机构地区： 宁波大学信息科学与工程学院宁波市新型功能材料及其制备科学国家重点实验室培育基地

出　　处： 《传感技术学报》 2009年第10期1371-1377,共7页

摘　　要： 研制了基于氯霉素抗体包被Fe3O4/Au金磁纳米微粒(GMP)和三乙撑四胺铜(Ⅱ)(CuL)共固定修饰平面热解石墨电极的安培免疫传感器(PRG|CuL/anti CAP-GMP),用于测定鱼肉中CAP含量。该免疫传感器是利用外加磁场,将antiCAP-GMP吸引到CuL修饰的PRG电极(PRG|CuL)表面制备而成。CuL对H2O2还原具有良好的电催化能力,当该传感器在含CAP样品液中温育后,CAP与电极表面的anti CAP的免疫结合物导致CuL对H2O2的催化还原电流(I)降低,电流下降值(△I)和CAP浓度成正比,可用于CAP定量测定。在25℃的pH=6.5磷酸盐缓冲液(PBS)中温育30 min,该传感器对CAP的检测线性范围为0.6～110 ng/mL,检出下限为0.092 ng/mL(3σ法)。应用于鱼肉中CAP检测并与传统的液相色谱法(HPLC)比较,结果一致,其添加回收率在97%～104%之间。该免疫传感器集分离、富集为一体,电极表面可更新,检测灵敏快速,对于水产品中痕量氯霉素分析提供了一种新颖的方法。 A magnetic nano particles modified amperous immunosensor for rapid separation-flee determination of chloramphenicol（CAP）in fish is proposed. The immunosensor is prepared by co-immobilizing CAP antibody（anti CAP） coated Fe3 04/Au magnetic nano-partieles （GMP） and triethylenetetramine copper （Ⅱ） （CuL）on a screen printed pyrogenation graphite electrode（PGE）through adding magnetic field absorption （PRG] CuL/anti CAP-GMP）. CuL can be employed as an electro-reductive catalyzation for H2O2. After the immunosensor is incubated with CAP solution at 25℃ for 30 min,the electron access between activity center of CuL and H2O2 is partly inhibited, which leads to a linear decrease of the catalytic efficiency of H2O2 by CAP in concentration ranges from 0.6 to 110 ng/mL. Under optimal conditions the detection limit for CAP immunoassay is 0. 092 ng/mL（3σ）. This sensor can be used to separate,enrichment and detection CAP with high sensitivity and easy to update its surface. This method is also promising in the detection of trace amount of CAP in aquatic products.

关 键 词： 三乙撑四胺铜 鱼肉 氯霉素 金磁纳米微粒 安培免疫传感器

领　　域： [理学] [理学] [自动化与计算机技术] [自动化与计算机技术]