作　　者： ; ; ; ; ; ; ;

机构地区： 华南理工大学轻工与食品学院

出　　处： 《食品与发酵工业》 2009年第9期88-92,共5页

摘　　要： 利用不同来源的蛋白酶催化玉米蛋白粉水解制备玉米蛋白肽,比较了不同水解度下所得产物肽的体外醒酒活性。结果表明,Alcalase为催化制备玉米粗肽的较理想催化剂。以水解度为指标,对Alcalase促玉米蛋白的水解条件进行了优化,确定底物质量浓度为5 g/mL、酶体积分数为1%、水解温度为60℃,水解时间为120min。在上述条件下,反应水解度可达48.4%,且反应水解度与肽提取率之间呈正相关性。Alcalase酶解产物玉米蛋白肽具有良好的体外醒酒活性,当控制酶解反应水解度为30%(肽提取率为57.0%)时,其ADH激活率可达38.5%。 Crude corn peptides were prepared via enzymatic hydrolysis of corn proteins by different proteases and their effects on the ADH activation ratios were compared. Among the examined proteases, Alcalase showed as the best catalyst, with which the enzymatic hydrolysis conditions were optimized. Results showed that the optimal enzyme dos- age, substrate concentration reaction conditions and reaction time were 1% , 5% , 60~C and 120min, respectively, under which the DH and peptide yield of the reaction reached 48.4% and 58.6% , respectively. Study of its antialco- holism activity in vitro showed that the highest ADH activation ratio was 38.5% when the DH and peptide yield reached 30.0% and 57.0% , respectively.

关 键 词： 玉米蛋白粉 玉米粗肽 酶解 激活率

领　　域： [轻工技术与工程] [轻工技术与工程] [轻工技术与工程] [轻工技术与工程]