作　　者： ; ; ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《植物遗传资源学报》 2009年第3期457-460,共4页

摘　　要： 香蕉束顶病毒(BBTV)是香蕉生产中的严重病害之一,主要通过感病材料和香蕉交脉蚜等昆虫进行传播,目前尚无有效防治方法。本研究以感染BBTV的巴西蕉(MusaAAA Cavendish)为材料,研究了感染BBTV的巴西蕉离体再生和超低温保存技术条件,表明离体茎尖在MS+6-BA4.0 mg/L+NAA0.4 mg/L的培养基上分化不定芽较好;采用玻璃化法超低温保存技术保存带有BBTV的香蕉茎尖,再生后植株BBTV脱除率达到60.6%,而常规的茎尖培养对BBTV的脱除率仅为26.7%。 Banana bunchy top virus （BBTV） is one of the most serious diseases of banana. It can cause great damage and there is no cure for BBTV. The virus can spread from plant to plant by aphids and from place to place by transporting infected materials. The utilization of cryopreservation for the eradication of banana bunchy top virus （BBTV） from banana （Musa spp. ） was investigated. This study was carried on banana cnltivar Baxijiao （Musa AAA Cavendish） , which infected by BBTV. The results show ed that the BBTV-infected shoot-tips differentiated well on the medium containing MS ＋ 6-BA 4.0 mg/L ＋ 0.4 NAA mg/L. Proliferating meristems were excised and then were cryopreserved through plant vitrification solution 2 （PVS2）. Up to 60.6% of the plants were free-BBTV after cryopreservation, while the conventional meristem tip culture resulted in only 26.7% BBTV-free plants.

关 键 词： 超低温保存 玻璃化法 香蕉束顶病毒 脱毒

领　　域： [生物学] [农业科学] [农业科学]