作　　者： ; ; ; ; ;

机构地区： 华南农业大学兽医学院

出　　处： 《广东畜牧兽医科技》 2009年第5期34-38,共5页

摘　　要： 本试验参考GenBank中已公布的新型鸭肝炎病毒全基因组序列,应用Primer Premier 5.0软件,在序列保守区域分别设计了7对引物,通过RT-PCR方法对本实验室已鉴别纯化的新型鸭肝炎病毒FS株进行了分段扩增,经过测序拼接整理,获得FS株全基因组序列(GenBank中登录号为EU877916)。序列分析表明,FS株全长7792个碱基,一个大的开放阅读框编码2251aa。将各基因与国内外已发表的新型鸭肝炎病毒参考毒株进行相似性分析,结果表明,与G株(GenBank登录号为EU755009)核苷酸相似性最高,为99.2%,而FS株与G株和C-GY株(GenBank登录号为EU352805)的氨基酸相似性最高,均为99.6%,与DHV-HS株和DHV-HSS株的相似性最低,均为83.6%。系统发育树分析发现,与G株亲缘关系最近。 The complete genome sequence of strain FS, a new serotype of duck hepatitis virus was determined by RT-PCR assay using seven pairs of primers designed with Primer Premier 5.0 software according to the conserved regions of complete genome sequence of the new serotype duck hepatitis virus deposited in GenBank. Sequence analysis showed that the genome of FS was 7792 nucleotides in size constituting one large open reading frame （GenBank accession number EU877916）. Compared with the sequences of the new serotype duck hepatitis virus in GenBank, the genome of FS had 99.2% identity with that of strain G, the amino acid sequences of FS had 99.6% identity with that of G strain and C-GY strain and had the lowest identity with that of strain DHV-HS and DHV-HSS. Phylogenetic analysis showed that it had the closest relationship with strain G.

关 键 词： 新型鸭肝炎病毒 全基因组 序列分析

领　　域： [农业科学] [农业科学] [农业科学]