作　　者： ; ; ; ; ;

机构地区： 广东海洋大学水产学院

出　　处： 《海洋与湖沼》 2009年第5期563-571,共9页

摘　　要： 对笛鲷属9种鱼的线粒体DNA16S rRNA基因片段进行了PCR扩增,扩增产物经纯化后测序,得到了长度为561bp的分析序列。结合GenBank中的另外9种笛鲷的16S rRNA同源序列计算得出A、T、G、C的含量平均为28.5%、22.1%、23.6%和25.8%,AT含量稍高于GC含量,18种笛鲷碱基组成差异不大。利用MEGA3.1软件对所得序列进行比对后检测到72个变异位点,其中包括简约信息位点44个,在变异位点中75.61%的碱基替换是由于发生了转换,转换/颠换平均为3.1︰1。计算了种间的遗传距离,结果表明,序列差异在0.0193-0.0680之间,其中勒氏笛鲷和金焰笛鲷、勒氏笛鲷和金带笛鲷的序列差异最小,而红鳍笛鲷和金焰笛鲷、红鳍笛鲷和画眉笛鲷的序列差异最大。选用高体四长棘鲷(Argyrops spinifer)为外群,利用NJ法构建了分子系统树,结果表明:本研究中的9种南海笛鲷与其它9种笛鲷进化关系较远;并且南海9种笛鲷相互之间仍然保持着着相对较远的遗传距离,遗传多样性较好。 MtDNA 16S rRNA genomic fragments of nine Lutjanus were amplified by PCR; and the purified product yielded a systematic sequence with 561bp being sequenced. The contents of A, T, G and C are respectively 28.5%, 22.1%, 23.6% and 25.8%. The contents of AT are slightly higher than those of GC, and the difference in base composition in 18 Lutjanus species is the minimum. Comparison with MEGA 3.1 software revealed 72 variation loci, of which 44 are information loci. For base replacement, 75.61% are caused by transition in it, and the ratio of transition/transversion is 3.1. The results of heredity distances computing show that the sequence difference is from 0.0193 to 0.0680, of which those of L. russelli and L. fulviflamma, L. russelli and L. vaigiensis are the smallest, while those of L. erythropterus and L. fulviflamma, L. erythropterus and L. vitta are the largest. By choosing Argyrops spinifer as outgroup and integrating the 16S rRNA homologous sequences of other nine Lutjanus from GenBank, the molecular phylogenetic trees with NJ method was constructed. The result shows that the nine Lutjanus in the research is away from the other nine＇s in terms of evolutionary relationship, and the nine Lutjanus from South China Sea keep relative long heredity distance between each other with obvious genetic diversity.

关 键 词： 笛鲷属 序列比较 分子系统学

领　　域： [生物学]