作　　者： ; ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《食品科学》 2009年第18期230-232,共3页

摘　　要： 采用高效液相色谱法测定金针菇中的多酚类成分,使用AgilentZORBAXSB-C18分析柱(4.6mm×250mm,5μm),流动相为乙腈(A)-0.4%乙酸(B),其中0～40min为5%A-95%B,40～45min为25%A-75%B,45～50min为35%A-65%B,之后运行5min,流速1.0ml/min,VWD检测器,检测波长280nm,柱温30℃。本研究在鲜金针菇中检出芦丁和槲皮素、在干金针菇中检出槲皮素和丁香酸。 A HPLC method was established to determine polyphenolic compounds in Flammulina velutipe fruitbody. The chromatographic separation was achieved on an Agilent Zorbax SB-C18 （4.6 mm × 250 mm, 5 μm） analytical column using a mobile phase consisting of a mixture of A： acetonitrile and B： 0.4% acetic acid （0-40 min： 5% A and 95% B; 40-45 min： 25% A and 75% B; 45-50 min： 35% A and 65% B） at a flow rate of 1.0 ml/min and 30 ℃ column temperature , and the analytes were detected using a VWD detector at 280 nm wavelength. Rutin and quercetin both were found in fresh Flammulina velutipe fruitbody, while quercetin, together with syringic acid was detected in dried samples.

关 键 词： 金针菇 芦丁 槲皮素 丁香酸 反相高效液相色谱法

领　　域： [轻工技术与工程] [轻工技术与工程]