作　　者： ; ; ; ;

机构地区： 肇庆学院生命科学学院

出　　处： 《安徽农业科学》 2009年第29期14175-14177,14206,共4页

摘　　要： [目的]为简便鉴定发根农杆菌菌株,比较其转化活力大小,筛选出高效转化黄瓜的发根农杆菌菌株。[方法]通过测定菌液生长曲线和进行各种生理生化试验,来初步鉴定供试农杆菌菌株和比较其生长活力;用供试农杆菌菌株对黄瓜子叶进行转化试验,以确定其是否为发根农杆菌并比较转化活力;用PCR方法对各供试农杆菌菌株进行分子鉴定。[结果]初步鉴定R1000、1.2556、11325、15834及R1025均为农杆菌,其转化活力从大到小依次为R1000(88.24%)、1.2556(52.08%)、15834(48.44%)、11325(36.17%)、R1025(33.33%);PCR结果可知,R1000、1.2556、11325、15834及R1025均含有发根农杆菌rolB基因。[结论]R1000为高效转化黄瓜的发根农杆菌菌株。 [ Objective ] The research aimed to provide a new approach for selecting Agrobacterium rhizogenes strain that could transformate cucumber （ Cucumis sativus L. ） efficiently. [ Method] A. rhizogenes strains （ R1000, 1. 2556, 11325, 15834 and R1025 ） were used as materials, traditional physiological and biochemical experiments were carried out. The growth curve of A. rhizogenes strains and transformation frequency of cucumber were measured. The gene rolB was detected by PCR. [ Result] All strains were A. rhizogenes ; The transformation frequency, from high to low, was R1000, 1.2556, 15834, 11325 and R1025; All the strains contained rolB gene ofA. rhizogenes. [Conclusion] R1000 was a highly efficient A. rhizogenes srtrain for cucumber transformation system.

关 键 词： 发根农杆菌 黄瓜 高效 菌株

领　　域： [农业科学]