作　　者： ; ; ; ; ; ;

机构地区： 河北农业大学食品科技学院

出　　处： 《安徽农业科学》 2009年第29期14032-14034,14115,共4页

摘　　要： ［目的］定性检测转基因大豆。［方法］以非转基因大豆和CP4-EPSPS转基因大豆为材料，以大豆内源基因Lectin和外源基因5-莽草酸-3-磷酸合成酶基因（CP4-EPSPS）为检测的目的片段，建立PCR反应体系。采用改进CTAB法提取大豆基因组DNA，并对所提DNA进行PCR扩增和电泳检测，确定转基因大豆的PCR检测限。［结果］改进CTAB法提取的大豆基因组DNA电泳条带清晰完整，转基因大豆和非转基因大豆基因组DNA均可扩增出约409 bp的条带即Lectin基因，而只在转基因大豆中检测出CP4-EPSPS特异性片段；当转基因大豆的含量为100%～0.2%时，均可扩增出特异性条带。［结论］该研究建立了转基因大豆的PCR检测方法。 [ Objective ] The study was to qualitatively detect the transgenic soybean. [ Method ] With non-transgenic soybean and CP4-EPSPS transgenic soybean as the materials and the endogenous gene （Lectin） of soybean and the foreign gene CP4-EPSPS as the tested fragments, PCR reaction system was established. The genomie DNA from soybean was extracted by improved CTAB method for amplification and electrophoresis detection, and the PCR detection limit on transgenic soybean was determined. [ Result ] The eleetrophoresis strips of genomie DNA from soybean extracted by improved CTAB method was clear and complete. Non-transgenic soybean and transgenic soybean all could amplified strips that about 409 bp （ Lectin gene） , but the specific strips CP4-EPSPS only were detected in transgenic soybean. When the content of transgenic soybean was 100% -0.2%, the specific strips all could be amplified. [ Conclusion] The study established the PCR detection method for transgenic soybean.

关 键 词： 转基因大豆 定性检测

领　　域： [农业科学]