作　　者： ; ;

机构地区： 云南农业大学动物科学技术学院

出　　处： 《云南农业大学学报》 1998年第3期301-305,共5页

摘　　要： 通过静脉注射的方法研究了大肠杆菌内毒素（ＥＴ）对山羊红细胞膜和肝粒体膜损伤的膜Ｃａ２＋－ＡＴＰ酶活性的变化及６５４－２（山莨菪碱）的保护效应．结果表明，ＥＴ处理组（Ⅱ组）在１ｈ，３ｈ红细胞膜Ｃａ２＋－ＡＴＰ酶活性高于对照组（Ⅰ组）（ｐ＜００５），５ｈ有下降趋势且持续到９ｈ．１２ｈ开始回升并低于Ⅰ组（ｐ＜００１，ｐ＜００５）．６５４－２处理组（Ⅲ组），Ｃａ２＋－ＡＴＰ酶活性除在９ｈ、１２ｈ与Ⅰ组，在３ｈ与Ⅱ组差异不显著（ｐ＞００５）外，均高于Ⅰ组和Ⅱ组（ｐ＜００１，ｐ＜００５）．在肝线粒体中，Ⅱ组Ｃａ２＋－ＡＴＰ酶活性在５ｈ、１２ｈ均低于Ⅰ组和Ⅱ组（ｐ＜００５，ｐ＜００１），Ⅲ组Ｃａ２＋－ＡＴＰ酶活性均高于Ⅰ组，但１２ｈ与Ⅰ组差异不显著（ｐ＞００５）．结果提示，ＥＴ具有诱导膜Ｃａ２＋－ＡＴＰ酶活性先激活后顿抑的效应，而６５４－２有明显保护膜Ｃａ２＋－ＡＴＰ酶活性的作用． The alterations of membrane Ca 2+ -ATPase activities of erythrocyte, hepatic mitochondria and protection of anisodamine(654-2) in goats were studied by intravenous injection of E. Coli (O 111 B 4) endotoxin (1 800 EU/kg body weight) and anisodamine (2 5 mg/kg body weight) alone as well as in combination. The results showed that endotoxin treatment (group Ⅱ)led to a marked increase of Ca 2+ -ATPase activities of erythrocyte membrane at 1 h and 3 h, a significant decrease of Ca 2+ -ATPase activities of erythrocyte membrane at 5 h, 7 h, 9 h, 12 h and liver mitochondria membrane at 5 h, 12 h in vivo, as compared to the normal control (group Ⅰ). Ca 2+ -ATPase activities in endotoxin and anisodamine coexposure group (group Ⅲ) were higher than those of group Ⅰand group Ⅱ, except no significant difference at 9 h, 12 h in erythrocyte membrane and at 12 h in liver mitochondria membrane as compared to grooup Ⅰduring all experiment. These results indicated that endotoxin could activate the membrane Ca 2+ -ATPase activities in the primary stage of administration of the endotoxin. Soon afterwards, Ca 2+ -ATPase activities were inhibited, whereas anisodamine had a prominent effect of protection on the Ca 2+ -ATPase of erythrocyte and hepatic mitochondria membrane of goats in vivo.

关 键 词： 内毒素 酶 线粒体膜 红细胞膜 山羊

领　　域： [农业科学] [农业科学]