作　　者： ; ; ;

机构地区： 中国农业大学农学与生物技术学院植物病理学系

出　　处： 《植物病理学报》 2009年第4期435-438,共4页

摘　　要： FM4-64,a kind of membrane-selective fluorescent dye,was utilized to stain the membrane structure of Botrytis cinerea and Phytophthora capsici.The effect of FM4-64 on hypha was observed under confocal microscope.The results indicated that the plasma membrane,speta and vesicles of the hyphae could be stained with FM4-64 at the concentrations of 1 to 20 μmol/L.The optimal dye concentration was 6 μmol/L for the both pathogens.At the same concentration of FM4-64,the staining of organelles was in a time-dependent manner and the different organelles were stained in diferrent times.The staining of plasma membrane and vesicles should be completed in 5 minutes,as other organelles can be stained after 10 minutes. FM4-64, a kind of membrane-selective fluorescent dye, was utilized to stain the membrane structure of Botrytis cinerea and Phytophthora capsici. The effect of FM4-64 on hypha was observed under confo- cal microscope. The results indicated that the plasma membrane, speta and vesicles of the hyphae could be stained with FM4-64 at the concentrations of 1 to 20 ixmol/L. The optimal dye concentration was 6 μmol/L for the both pathogens. At the same concentration of FM4-64, the staining of organelles was in a time-dependent manner and the different organelles were stained in diferrent times. The staining of plasma membrane and vesicles should be completed in 5 minutes, as other organelles can be stained after 10 minutes.

关 键 词： 染色效果 囊泡运输 植物病原真菌 应用 细胞膜 卵菌 亚细胞定位 二溴化物

领　　域： [生物学] [轻工技术与工程]