作　　者： ; ; ; ;

机构地区： 西北农林科技大学植物保护学院陕西省农业分子生物学重点实验室

出　　处： 《微生物学通报》 2009年第9期1345-1349,共5页

摘　　要： 本研究从秸秆中分离得到一株弹性蛋白酶高产菌,并对该菌株进行鉴定,以期为实现其工业化生产提供理论依据。采用酪蛋白(脱脂奶粉)进行初筛后,以弹性蛋白(牛筋)进行复筛,并对筛选结果进行检验,然后对所得菌株结合形态、生理生化特性和16SrRNA基因序列进行分类鉴定,得到一株弹性蛋白酶高产菌株LSF-97。结果显示,菌株LSF-97与短小芽孢杆菌同源率达到100%,形态及生理生化特征与模式菌也显示高度一致性,故将其确定为短小芽孢杆菌。 The study provide a theoretical basis for the industrial production of a elastase-high-yield strain which was isolated from the straw, and the selected strain was identified. The screening strategy included casein （skim milk） plate selecting and elastin （beef tendon） re-screening. And then, the morphological, physiological and biochemical characteristics as well as 16S rRNA sequence homology of the selected strain were studied. Finally, the strain LSF-97 which had a excellent decomposing ability to beef tendon was obtained. The results showed that the strain LSF-97 is relative to the Bacillus pumilus with 100 % similar in sequence under the phylogenetic tree, the morphological and physiological and biochemical characteristics are also consistent with pattern of bacteria. So it was identified as Bacillus pumilus.

关 键 词： 芽孢杆菌 蛋白酶 筛选 鉴定

领　　域： [轻工技术与工程] [化学工程] [轻工技术与工程]