作　　者： ; ;

机构地区： 海南大学农学院

出　　处： 《中国农学通报》 2009年第18期417-419,共3页

摘　　要： 采用改进CTAB法分别提取狗牙根(Cynodon dactylon)、结缕草(Zoysia spp.)、假俭草(Eremorchloa ophiuroides)3种暖季型草坪草基因组DNA。得到的DNA经核酸蛋白含量测定仪测得A260/A280值处于1.74～1.98之间、A260/A230集中处于1.74～1.99,经琼脂糖凝胶电泳检测各样品具有一条明亮主带并无拖尾现象。表明改进CTAB法提取得到的DNA完整性好,纯度高,适用于分子生物学的研究。 Genome DNA was extracted by the improved method from leaves of Cynodon dactylon（L.） Pers, Zoysia spp, Eremorchloa ophiuroides. The detection performed on a nucleic acids and protein analyzer showed that A260/A280 was situated betweenl.74-1.98,A260/A230 was ranged from 1.74 to 1.99.The extracted products had a bright band and without tailing bands which could be observed through agar gel electrophoresis. These results showed that genomic DNA obtained by optimum method which had a good integrity and high purity, and could be used in each kind of molecular biology research.

关 键 词： 暖季型草坪草 基因组 提取

领　　域： [农业科学]