作　　者： ; ; ; ; ;

机构地区： 深圳市第二人民医院

出　　处： 《山东医药》 2009年第29期20-21,共2页

摘　　要： 目的探讨过氧化物酶增殖体激活受体γ辅激活因子1α(PGC-1α)对高糖状态下血管内皮细胞线粒体生物合成的影响。方法体外原代培养人脐静脉内皮细胞,分别放置于正常糖浓度(对照组)和高糖浓度(高糖组)培养液中。用含有编码PGC-1α蛋白基因的腺病毒表达载体及空壳载体分别感染上述两组细胞,采用Southern印记法以细胞色素b DNA为探针检测线粒体DNA拷贝数。结果与对照组相比,高糖组线粒体DNA拷贝数显著下降;通过质粒转染过度表达PGC-1α导致细胞总DNA中细胞色素b DNA升高,线粒体生物合成增加。结论内皮细胞中过量表达的PGC-1α可以显著促进线粒体生物合成,这一作用在高糖培养的内皮细胞中更加明显。 Objctive To investigate the effect of peroxisome proliferator activated receptor gama coactivator 1 alpha（PGC-1α） on mitochondrial biogenesis of endothelial cells in hyperglycemia.Methods Human umbilical vein endotheial cells（HUVECs） were isolated from human umbilical veins and cultured in DMEM medium with normal sugar density（control group） and high sugar density（hyperglycemia group）.Adernovirus expression vector with encoding PGC-1α protein and adernovirus expression vector without encoding PGC-1α protein were transfeeted into the above cells. Mitochondrial biogenesis was evaluated by Soutbem-blottlng using eytochrome b cDNA probe. Results Compared with control group, Mitochondrial DNA decreased in the endothelial cells in high glucose group, and over expression of PGC-1α resulted in the increase of eytochrome b DNA of general DNA in cells and the stronger mitochondrial biogenesis. Conclusions Over expression of PGC-1α in ceils, espeeially in endotheliocytes cultured in DMEM medium with high sugar highly increases mitochondrlal biogenesis.

关 键 词： 过氧化物酶增殖体激活受体 辅激活因子 血管内皮细胞 线粒体

领　　域： [生物学]