作　　者： ; ; ; ;

机构地区： 中国矿业大学化工学院

出　　处： 《福建林业科技》 2009年第3期117-120,140,共5页

摘　　要： 探讨了抗氧化剂柠檬酸、抗坏血酸(VC)、植酸(PA)和聚乙烯吡咯烷酮(PVP),吸附剂活性炭(AC)对银杏愈伤组织继代培养中细胞生长和褐化的影响,结果表明:柠檬酸0.2 g.L-1对银杏继代细胞生长速率促进最大,可达11.354%;15 mg.L-1VC抗褐化效果最好,叶绿素含量可达0.248%。综合细胞生长和抗褐化效果,最佳处理为柠檬酸0.2 g.L-1。 The effects of five antioxidants and sorbent, including citric acid, VC, PA and PVP, AC, on cell growth and browning in ginkgo callus generation-continuing culture were discussed in this article. The results showed that：The growth rate, 11. 354 %, of ginkgo generation-continuing cell was mostly influenced by 0.2 g. L^-1 of citric acid;The best aiti-browning substract was 15 mg . L^-1 of VC. Chlorophyl content was 0. 248 %. Synthetically considering the cell growth and aiti-browning effects, the best treatment was 0.2 g. L^-1 of citric acid.

关 键 词： 银杏 抗氧化剂 吸附剂 愈伤组织 细胞生长 细胞褐化

领　　域： [农业科学] [农业科学]