作　　者： ; ;

机构地区： 华南农业大学动物医学系

出　　处： 《中国兽医学报》 1998年第5期490-493,共4页

摘　　要： 将３６头杂交山羊随机分成３组：Ⅰ组作为对照；Ⅱ组静注大肠杆菌内毒素１８００ＥＵ／ｋｇ，注射２次，间隔２４ｈ；Ⅲ组在同Ⅱ组处理基础上，于第２次注射前１０ｍｉｎ静注山莨菪碱（６５４－２）注射液２．５ｍｇ／ｋｇ。第２次注射内毒素后５ｈ和１２ｈ各组均剖杀６头山羊，检测肝脏抗氧化系统功能。结果表明：Ⅱ组肝组织丙二醛（ＭＤＡ）含量明显高于Ⅰ组（Ｐ＜０．０５），谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）和谷胱甘肽还原酶（ＧＲ）活性及总超氧化物岐化酶（Ｔ－ＳＯＤ）、Ｍｎ－ＳＯＤ活性显著降低（Ｐ＜０．０１，Ｐ＜０．０５），同时肝组织还原型谷胱甘肽（ＧＳＨ）、总巯基（Ｔ－ＳＨ）、非蛋白巯基（ＮＰ－ＳＨ）和蛋白结合巯基（ＰＢ－ＳＨ）含量下降（Ｐ＜０．０５）。而Ⅲ组肝组织ＭＤＡ含量于５ｈ时与Ⅰ组无显著差异（Ｐ＞０．０５），１２ｈ明显低于Ⅱ组（Ｐ＜０．０５），ＧＳＨ－Ｐｘ和ＧＲ活性在５ｈ下降不显著，ＧＲ活性至１２ｈ高于Ⅱ组（Ｐ＜０．０５），Ｔ－ＳＯＤ、ＣｕＺｎ－ＳＯＤ活性和ＧＳＨ、Ｔ－ＳＨ、ＮＰ－ＳＨ、ＰＢ－ＳＨ含量均显著高于Ⅱ组（Ｐ＜０．０５）。提示山羊内毒素休克时肝脏产生明显的氧自由基诱导的脂质过氧化损伤，提前注射６５４－２能够有效地减? Thirty-six hybrid goats were divided randomly into control group (Ⅰ), endotoxic shock group (Ⅱ) with intravenuous injection of E coli endotoxin (1800 endotoxin unit per kilogram body weight, 24 hours apart) and anisodamine pretreated group (Ⅲ). The liver antioxidant functional parameters were examined at 5 h and 12 h postinjection respectively. The liver MDA contents of group Ⅱ were found significantly increased corresponding in time to the reduction of activities of glutathione peroxidase and glutathione reductase, total superoxide dismutase(T SOD), Mn SOD(P<0.01, P<0.05). Meanwhile, the levels of liver reduced glutathione(GSH), total sulfhydryl group, protein bound(PB SH) and nonprotein bound sulfhydryl group (NP SH) also markedly decreased in group Ⅱ at 5 h and 12 h postinjection. However, the liver MDV contents of group Ⅲ were not increased at 5 h postinjection and were significantly lower than those of group Ⅱ at 12 h postinjection. At 5 h postinjection, the activities of liver GSH Px and GR did not decrease significantly as compared with group Ⅰ. The activities of liver GR were higher than those of group Ⅱ(P<0.05). There were no differences of the activities of T SOD, and the levels of liver tissue T SH, NP SH and PB SH between group Ⅰ and Ⅲ. These data revealed the role of free radicals in the pathophysiology of endotoxic shock suggesting that the anisodamine given 10 min prior to endotoxin injection can effectively attenuate the lipid peroxidation.

关 键 词： 内毒素休克 自由基 山莨菪碱 肝脏 山羊

领　　域： [农业科学] [农业科学] [农业科学]