作　　者： ; ; ; ; ; ; ; ;

机构地区： 华中农业大学动物医学院农业微生物学国家重点实验室

出　　处： 《中国兽医学报》 2009年第9期1125-1128,共4页

摘　　要： 犬血样经Giemsa染色镜检及PCR检测，证实感染附红细胞体。选择添加新生牛血清（BS）、酵母浸出液、葡萄糖等成份的PPLO肉汤（PPLO—S—BS）为培养液，将染虫红细胞稀释后接种到细胞培养板，置37℃、5％CO2的培养箱中培养。24～72h后红细胞溶血变为带虫血影（ghost）；在PPLO-S-BS中虫体生长较好，并能连续培养180d；除首次接种外不添加任何红细胞。对PPLO-S-BS中培养的带虫血影进行了光镜及扫描电镜观察、PCR鉴定、感染阴性红细胞试验、抗生素敏感性试验，结果表明其特征与接种物相同。 Canine blood samples were confirmed positive for Haemomycoplasma by Giemsa stained blood smears as well as by PCR. Experiments were conducted to develop an in vitro cultivation of the organisms from the infected blood samples. A PPLO broth medium supplemented with fetal calf serum, yeast extract,and glucose was selected for canine Haemomycoplasma cultivation. The culture was incubated in an atmosphere of 5 % CO2, in air at 37℃ for 180 days;The infected red blood cells became ghosts with parasites after 24-72 h of cultivation. No any host erythrocytes were added during the maintenance except initiation. The cultured organisms adhering to ghosts were identified as Haernomycoplasma by light and electron microscopic examinations,identical gene sequences, infecting negative RBCs tests, and antibiotics sensitivity tests.

关 键 词： 嗜血性霉形体 附红细胞体 犬 肉汤 体外培养 血影

领　　域： [农业科学] [农业科学] [农业科学]