作　　者： ; ; ; ; ;

机构地区： 中国科学院过程工程研究所生化工程国家重点实验室

出　　处： 《过程工程学报》 2009年第4期763-769,共7页

摘　　要： 以二乙烯基苯为原料,采用微孔膜乳化法制备了尺寸均一的乳液,在75℃下悬浮聚合24h,制得均一的聚二乙烯基苯微球,对其表面形态、粒径及其分布进行了表征,并考察了微球的色谱分离性能.结果表明,微球呈多孔球体结构,粒径约为25μm,平均孔径为14nm,比表面积达520m2/g,且机械强度好(可耐40～50MPa高压).利用该微球装填的半制备级高效液相色谱柱(300mm×10mmI.D.)分别分离酚类混合物和多肽混合物,分离度均大于1.5,最大理论塔板数分别达13000和21000m-1,批次间重现性良好. Taking divinylbenzene as monomer,polydivinylbenzene（PDVB） microspheres with particle size 25 μm were prepared by membrane emulsification and suspension polymerization at 75℃.Their morphology and size distribution of pores were investigated,their application in reversed-phase chromatographic separation of small molecular compounds and peptides examined.The prepared PDVB microspheres had uniform particle size（span value=0.91）,mean pore size of 14 nm,specific surface area of 520 m2/g,and high mechanical intensity（keeping stable under 40～50 MPa pressure）.The PDVB microspheres were packed into semi-preparative columns（300 mm×10 mm I.D.） and tested by separating phenol derivatives and peptides mixture on high performance liquid chromatography.The resolution of both systems was higher than 1.5,and theoretical plate number reached 13000 and 21000 m-1,respectively.PDVB microspheres showed perfect batch to batch reproducibility on separation.They would be a promising reversed-phase stationary phase for preparative and process purification of peptides.

关 键 词： 聚合物介质 制备 高效液相色谱 多肽 分离

领　　域： [化学工程] [理学] [理学]