作　　者： ; ; ; ; ;

机构地区： 仲恺农业工程学院生命科学学院

出　　处： 《华北农学报》 2009年第4期74-79,共6页

摘　　要： 为阐明高渗透压甘油激酶在链格孢菌中的功能,通过遗传学手段,构建了细极链格孢菌(Alternaria tenuissi-ma)cDNA酵母表达文库,并对其进行筛选而获得细极链格孢菌高渗透压甘油蛋白激酶AtHOG1基因,该基因全长1 539 bp,编码355个氨基酸。AtHog1p含有MAPK保守的激酶激活区域"TGY",与来源于烟曲霉(Aspergillus fumigatus)AfOsm1p(XP-752664)、稻瘟菌(M.grisea)MG01822(XP-363896)及酿酒酵母(S.cerevisiae)ScHog1p(AAB67558)高度同源,相似性分别为94%,90%和80%。在高渗环境下,AtHOG1基因与ScHOG1基因功能相同。链格孢菌中存在HOG通路信号途径,AtHOG1基因可能与链格孢菌的逆境适应性调节密切相关。 An Alternaria tenuissima cDNA expression library was constructed. The MAP kinase HOG1 was isolated from the Alternaria tenuissima cDNA expression library, designated as AtHOG1. It had a size of 1 539 bases in length, encoded a protein of 355 amino acids. The AtHoglp contained the conserved TGY activation loop found in the stress-activated protein kinase subgroup of MAPKs and its amino acid sequence showed 94% ,90% and 80% identities with MOsmlp （XP-752664） of Aspergillus fumigatus, MgO 1822 （XP-363896） of Magnaporthe grisea and ScHogl p （AAB67558） of Saccharomyces cerevisiae, respectively. AtHOG1 cDNA sequences could complement the functions of S. cerevisiae ScHOG1 genes in sodium chloride tolerance, suggesting a functional HOG pathway exists in A. tenuissima, AtHOG1 gene was involved in the stress adaptation regulation of A. tenuissima.

关 键 词： 高渗透压甘油蛋白激酶 细极链格孢菌 基因 逆境胁迫调节

领　　域： [生物学]