机构地区: 广东海洋大学水产学院广东省水产经济动物病原生物学及流行病学重点实验室
出 处: 《渔业现代化》 2009年第4期37-41,共5页
摘 要: 从患病方斑东风螺(Babylonia areolata)分离到一株致病菌Balo001,运用生理生化方法并结合分子生物学方法对病原菌进行分类鉴定,并对病原菌进行药敏分析及半致死剂量(LD50)测定。结果表明:致病菌Ba-lo001为哈氏弧菌(Vibrio harveyi),它对东风螺的LD50值为6.8×105CFU/g;该菌对氟哌酸、氟苯尼考、复方新诺明等抗菌药有较高的敏感性。 An epidemic proboscis edema disease of Babylonia areolata occurred in Zhanjiang in October of 2005. A pathogen named Balo001 isolated from moribund Babylonia areolata by aseptic methods, we have made use of physiological and biochemical methods combined with molecular biology methods to classify and identify the pathogen, and drug sensitivity analysis of pathogens as well as LDs0 value were tested too. The resuits showed that the pathogen named Balo001 was Vibrio harveyi. The LDs0 of the pathogen to Babylonia areolata was 6.8 × 10^5CFU/g. Antibiotic sensitivity analysis indicated that the pathogen was sensitive to norfloxacin,florfenicol, compound Sulfamcthoxazolc. This showed that the pathogenic bacteria has strong toxiferous to Babylonia areolata, it can lead to the proboscis edema disease of Babylonia areolata. The results of the research provide a theoretical basis for prevention and control of the proboscis edema of Babylonia areolat.