机构地区: 福建农林大学
出 处: 《基因组学与应用生物学》 2009年第4期640-644,共5页
摘 要: 本实验从花生果皮种仁抑制消减文库中筛选出了一个255bp的EST序列并进行了研究。构建了花生果种皮全长cDNA库并从中筛选出该基因的全长,命名为AhPSG8。此基因全长1118bp,开放阅读框第33~833个碱基,编码266个氨基酸残基组成的多肽。由生物信息学分析表明该基因编码多个活性位点蛋白,可能与细胞内DNA的转录有关;结构分析揭示出AhPSG8具有一个跨膜区,N端是一个由20个氨基酸组成的信号肽;该基因与已发表的基因序列没有明显的同源性,为一新基因;RT—PCR研究该基因在花生中的表达,结果显示该基因在果种皮中特异表达,10~40d果皮中丰富表达,推测为与花生果种皮发育相关基因。 A 255 bp EST was isolated from the suppression subtractive hybridization (SSH) library of kernel and pericarp in this research, we constructed a full length pericarp and testa cDNA library, and the full length of the new gene was obtained, which was named A hPSG8, its full-length was 1 118 bp with an open reading frame from 33 bp to 833 bp. Bioinformatics analysis showed that the deduced protein contains a cross-membrane region and many active loci, which suggest its transcript action in cell. Also, the protein has a signal peptide containing 20 amino acids. This gene is a novel one which has no significant similarity with the sequences published. Expression analysis by RT-PCR revealed that this gene was specially expressed in pod and testa and largest in amount in pericarp from 10 to 40 days.