作　　者： ; ; ; ;

机构地区： 深圳市仙湖植物园

出　　处： 《热带亚热带植物学报》 2009年第4期383-387,共5页

摘　　要： 以吊钟花（Enkianthus quinqueflorus Lour.）茎尖及带腋芽的茎段为外植体进行组织培养。结果表明，改良B5培养基（B5大量元素和钙盐＋MS有机物、铁盐、微量元素）最有利于吊钟花的培养，外植体在改良B5＋2，4-D1mgL^-1的培养基中，愈伤组织诱导率可达100％。在含BA1～2mgL^-1＋NAA0．1～0．5mgL^-1培养基中，可诱导产生不定芽。继代培养以改良毯＋BA1mgL^-1＋NAA0．5mgL^-1培养基的增殖系数最高。生根培养基以1／2MS＋IBA2mg L^-1为最佳，生根率可达80％以上。试管苗移栽成活率为90％以上。 The rapid propagation of Enkianthus quinqueflouus Lour. had been established by using shoot-tip or stem with auxiliary buds as explants. The modified B5 medium as basal medium was optimum for callus induction. The callus induction rate was up to 100% cultured on Bsmedium with 1 mg L^-1 2,4-D. The adventitious buds were induced on 135 medium supplemented with 1- 2 mg L^-1BA ＋ 0.1 -0.5 mg L^-1 NAA. Propagation coefficient was the highest when adventitious buds were subcultured on modified 135 ＋ 1 mg L^-1 BA ＋ 0.5 mg L^-1 NAA. The rooting could reach 80% when adventitious buds cultured on 1/2MS ＋2 mg L^-1 IBA, and the survival of plantlet could reach 90% after transferred into perlite bed.

关 键 词： 吊钟花 组织培养 茎

领　　域： [生物学]