作　　者： ; ; ; ; ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《茶叶科学》 2009年第4期251-256,共6页

摘　　要： 采用Hoechst33258荧光染色法观察EGCG、Zn2+和EGCG+Zn2+对前列腺癌PC-3细胞凋亡的诱导作用;采用HPLC法检测了EGCG、Zn2+和EGCG+Zn2+对PC-3细胞内腺苷酸(ATP、ADP、AMP)含量的影响,并分析了能量负荷(EC)和ATP/ADP比值。结果表明,Hoechst33258染色后正常细胞发出均匀微弱的蓝色荧光,细胞核未见异常,EGCG、Zn2+和EGCG+Zn2+处理后凋亡的PC-3细胞都发出较强的蓝色荧光,细胞核DNA断裂及染色体高度浓缩;EGCG、Zn2+及二者混合物处理后细胞内总腺苷酸含量、EC及ATP/ADP比值都下降,表明EGCG与Zn2+对PC-3细胞能量代谢都存在明显的抑制作用。 In the present work, the effect of treatments with EGCG, Zn^2＋ and EGCG＋Zn^2＋ on inducing apoptosis and content of adenylate nucleotide （ATP, ADP and AMP） in PC-3 cells was investigated by chromatin staining with Hoechst 33258 and HPLC. Hoechst 33258 staining revealed the appearance of condensed chromatin and fragmented apoptotic nuclei under fluorescence microscopy at a concentration of 80 μmol/L EGCG, 80 lamol/L Zn^2＋ and 80 μmol/L EGCG＋80 μmol/L Zn^2＋ in PC-3 cells. In the determination of adenylate nucleotide, after treated with EGCG, Zn^2＋ and EGCG＋Zn^2＋, content of total adenylate nucleotide, EC and ATP/ADP value were significantly decreased in PC-3 cells, which demonstrated that EGCG and Zn^2＋ have a markedly inhibitory effect on energy metabolism of PC-3 cells.

关 键 词： 锌离子 细胞 细胞凋亡 腺苷酸 能量代谢

领　　域： [农业科学] [农业科学] [生物学]