机构地区: 中国林业科学研究院森林生态环境与保护研究所国家林业局森林生态环境重点实验室
出 处: 《现代生物医学进展》 2009年第13期2484-2486,共3页
摘 要: 方便高效的保存方法是开展动物肠道微生物研究的重要前提,用T-RFLP(末端限制性片段长度多态性)结合16S rDNA分析评估了野外采样常用的DETs(20%DMSO,0.25 M sodium-EDTA,100 mM Tris,pH 7.5,and NaCl to saturation)缓冲液对不同保存时间(24h,7天,30天,90天)粪便样品肠道微生物DNA的保存效果。结果表明,DETs保存在30天保存时间内微生物群落结构无显著变化,95.6%的T-RFs仍然存在,能效好的保护肠道微生物DNA。 Convenient and effective preservative methods are important to intestinal microbes community research. We evaluated the effectiveness of DETs buffer (20% DMSO, 0.25 M sodium-EDTA, 100 mM Tris, pH 7.5, and NaCI to saturation) for intestinal microbes DNA Preservation. Effectiveness was evaluated by T-RFLP (Terminal restriction fragment length polymorphism) combined with 16S rDNA analysis at time points of 24 hours, 7 days, 30 days, 90 days.The results showed that DETs buffer could preserve samples well for 1 month (95.6% peaks were kept, means).
领 域: [生物学]