作　　者： ; ; ; ; ; ;

机构地区： 广东海洋大学水产学院

出　　处： 《水产科学》 2009年第7期378-382,共5页

摘　　要： 以溶藻弧菌为研究对象,对定量检测生物被膜的超声波平板法进行条件优化,对优化的超声波平板法与平板擦拭法进行比较,并采用银染法进行验证。试验结果表明,优化的超声波平板法的最佳条件为:超声波功率120 W,处理14 min,处理温度25℃,优于平板擦拭法。 Biofilms are characterized by widespread and hard to be dete tential risk to aquaculture production and fishery product safety. The ed and eliminated,which are poltrasonic-plate method was optimized to eliminate bacterium Vibrio alginolyticus as a biofilms model strain and compared with the plateswabbing method and then the silver-staining method was used for verification. The results showed that the optimal conditions for ultrasonic-plate method were found to be： ultrasonic power of 120 W, treatment time 14 min and temperature of 25 ℃. The optimized ultrasonic-plate method was superior to the plateswabbing method.

关 键 词： 生物被膜 溶藻弧菌 超声波平板法 优化条件

领　　域： [农业科学] [农业科学]