作　　者： ; ; ; ;

机构地区： 韩山师范学院

出　　处： 《北方园艺》 2009年第7期62-64,共3页

摘　　要： 用橄榄成熟胚作为外植体,诱导胚长成无菌苗,在成熟芽苗上诱导多分枝侧芽及生根,筛选出MS+6-BA 0.5+KT 1+NAA 0.5为诱导多分枝侧芽的适合培养基,取得92%的侧芽诱导率;MS+NAA 1+IBA 1为生根培养基,取得88%的生根率,成功培育出带多分枝侧芽的橄榄苗,为橄榄栽培的矮化密植、提高产量奠定基础。应用固-液培养基长效抑制培养物褐变,此方法对于生物碱含量较高、培养过程容易产生褐变的植物的离体培养有一定的借鉴作用。 The embryo of olive, used as explant, was induced to grow up to be asepsis shoots, and cut the mature shoots for the inducement of lateral bud and radication. MS＋6-BA 0. 5 ＋KT 1 ＋ NAA 0. 5 and MS＋NAA 1 ＋IBA 1 were screened out as the effective substrate of lateral bud abduction and radieation cultivating, and acquired lateral bud abduc- tion rate which was up to 92% and radication rate 80% respectively, and successfully to cultivate the olive seedlings with muhi-lateral bud. It established the base of using plant clone to propagate technical-breeding olive seedling rapidly. Moreover, the method of applying solid-liquid medium to inhibit cultures browning had referential experience for the excised culture of the plant which was with higher alkaloid content and prone to browning during the process.

关 键 词： 橄榄 多分枝侧芽 矮化 固 液培养基

领　　域： [农业科学] [农业科学]