作　　者： ; ; ; ; ; ; ;

机构地区： 华南理工大学轻工与食品学院

出　　处： 《陕西科技大学学报（自然科学版）》 2009年第3期32-39,共8页

摘　　要： 使用一种简单的两步沉淀方法研究了Mg2+和Ca2+对大豆球蛋白及β-伴球蛋白级分分级的影响,并对所得产品的某些理化性质进行了表征.结果表明,加入5mMMg2+是优化的条件;使用5mMMg2+得到的大豆球蛋白和β-伴球蛋白级分的植酸含量分别是0.4%和1.3%;加入5mMCa2+却使球蛋白级分植酸含量较高,为1.3%;植酸-镁复合物要比植酸-钙复合物的溶解性要高许多;使用Mg2+所获得的β-伴球蛋白级分的热稳定性获得了提高,使用Mg2+得到的富含球蛋白的级分的可溶性在pH<4.5下显著高于使用Ca2+得到的相应级分.排阻色谱(SEC-HPLC)实验结果表明高植酸含量会引起球蛋白的聚合. A simple two step precipitation method was used to investigate the effect of Mg^2＋ instead of Ca^2＋ on fractionation of soybean glycinin and β-conglycinin. Compositional and physicochemical properties of the resulting protein fractions were characterized. The optimized procedure was obtained when the addition of 5 mM Mg^2＋ was used in terms of protein yield, purity, phytate content and physicochemical property. After application of 5 mM Mg^2＋, the phytate content of the glycinin-rich and fl-conglycinin-rich fractions was about 0.4% and 1.3%, respectively, but the addition of 5 mM Ca^2＋ resulted in a higher phytate content in the glycinin-rich fraction. Furthermore, the solubility of the glycinin-rich fractions by Mg^2＋ was significantly higher than that by Ca^2＋ at pH 〈 4. 5. In addition, application of mM Mg^2＋ improved thermal stability of the β-conglycinin-rich fraction. Size exclusion liquid chromatography analysis suggested further that high phytate content might cause the aggregation of glycinin globular.

关 键 词： 植酸 钙 镁 大豆蛋白分级 球蛋白 伴球蛋白

领　　域： [轻工技术与工程] [轻工技术与工程]