作　　者： ; ; ; ;

机构地区： 南昌大学

出　　处： 《食品科学》 2009年第13期18-21,共4页

摘　　要： 本实验采用荧光光谱、紫外-可见光谱和盐效应等手段研究荷叶碱与小牛胸腺DNA(ct-DNA)之间的相互作用,并在pH7.4的Tris-HCl介质中,以中性红为荧光探针,对其作用机理进行研究。结果表明:在生理条件下(pH7.4),荷叶碱与DNA发生作用方式为混合方式,嵌插与沟槽作用是两种主要作用方式,DNA对荷叶碱荧光猝灭属于静态猝灭,测得其结合常数为1.396×106L/mol。 The interaction between nuciferine and ct-DNA was determined by the means of fluoresence spectrometry and UV- VIS spectrometry, and the effects of NaCI and KI on the interaction were investigated. The mechanism of the interaction was also discussed by fluorescence spectroscopy using neutral red （NR） as a spectral probe in Tris-HCl buffer solution （pH 7.4）. The results suggested that under physiological condition （pH 7.4） nuciferine could interact with DNA duplexes in a minor-groove binding mode or an intercalative mode. Fluorescence quenching studies revealed the presence of a static component in the quenching mechanism. The quenching constant for the interaction between nuciferine and ct-DNA was 1.396 × 10^6 L/mol.

关 键 词： 荷叶碱 小牛胸腺 中性红探针 荧光光谱 嵌插方式

领　　域： [理学] [理学]