作　　者： ; ; ; ; ; ; ;

机构地区： 南方医科大学

出　　处： 《畜牧兽医学报》 2009年第6期800-805,共6页

摘　　要： 通过扩增102头西藏小型猪以及16头巴马小型猪、17头贵州香猪的线粒体DNA控制区,测序并与国内其他猪种进行比较,研究西藏小型猪的遗传标记以及与其他国内地方猪种的亲缘关系。结果显示,西藏小型猪线粒体DNA D-loop区分3个区域。串联重复序列区处于中间位置,包含有15~29个10 bp的重复片段,分为A、B 2种类型。3′端340 bp,与国内其他猪种的序列相同,比较保守 D-loop 5′端704 bp,共有22个变异位点。由22个变异位点中归纳出25个单倍型,其中有2种主要的单倍型,分别占34.4%和36.6%。根据3个转换位点：305、500、691,将西藏小型猪分成了2组,几乎与串联重复序列所分的A、B 2组类型相对应。与西藏小型猪相比,巴马小型猪和贵州香猪D-loop 5′端变异位点较少,分别只有4种和2种单倍型,串联重复区也只有1个类型。说明西藏小型猪可能有2个母系祖先,并且与我国西南地区的猪种有较近的亲缘关系 不同的串联重复片段类型和5′端的变异位点可以联合组建西藏小型猪的遗传标记。 To analyze mitochondrial DNA D-loop region sequences of Tibet Mini-pigs,and to ex- plore their cytoplasmic DNA markers used to identify them and the phylogenetic relationship be- tween Tibet Mini-pigs and other local pig breeds in China, mtDNA D-loop region in 102 Tibet Mini-pigs, 16 Bama miniature pigs and 17 Guizhou Xiang pigs were amplified and sequenced, fol- lowed by comparing with the data of other Chinese domestic pigs. The result showed that the D- loop region of Tibet Mini-pigs was divided into three areas. The tandem repeat sequence which had 15-29 10 bp repeat motives and located in the middle of mtDNA D-loop region consisting of type A and B. 340 bp sequence at 3＇ end of the D-loop which was very conservative exhibited par- ticularly high homology with the same region of other domestic pigs. 704 bp sequence at 5＇ end of the D-loop had 22 polymorphic sites. 22 polymorphic sites deduced 25 haplotypes, among which the percentage of two major haplotypes reached 34. 4% and 36.6%, respectively. Based on three transform sites （305, 500, 691）, Tibet Mini-pigs were divided into two groups, nearly corre- sponding to type A and B of tandem repeat sequence. Compared with Tibet Mini-pigs, Bama min- iature pigs and Guizhou Xiang pigs had four and two haplotypes, respectively, and had type A of tandem repeat sequence. It is inferred that Tibet Mini-pigs originated from two maternal ancestors and were close relative relationship with local pigs in southwest China. The repeating motif in combination with the variable sites at the 5＇ end of mtDNA D-loop region can be uesd as the genetic marker to identify Tibet Mini-pigs.

关 键 词： 西藏小型猪 线粒体 控制区 遗传标记 亲缘关系

领　　域： [农业科学] [农业科学] [生物学]