作　　者： ; ; ; ;

机构地区： 浙江林学院林业与生物技术学院浙江省现代森林培育技术重点实验室

出　　处： 《林业科学》 2009年第6期34-38,179,180,共7页

摘　　要： 利用光学显微镜和透射电子显微技术研究山核桃本砧嫁接过程中的愈伤反应。结果表明:砧、穗形成层区域最先形成隔离层,嫁接后第1～3天形成隔离层,第3～6天逐渐加厚,随着隔离层细胞内物质逐渐被吸收,愈伤组织细胞分裂后突破隔离层,至嫁接后第12天隔离层完全解体消失;隔离层两侧愈伤组织细胞进行旺盛的生理代谢活动,切口外侧细胞的胞间连丝迅速关闭,而内侧细胞间胞间连丝物质交换活跃;近隔离层的细胞之间部分细胞壁破裂,细胞间的原生质体发生融合;线粒体、内质网、质体数量显著增加,多泡体、壁旁体等膜状结构频繁出现,淀粉粒先积累后逐渐消失。 A shoot homoplastic graft in Carya cathayensis was performed and the healing responses were observed with light microscope and transmission electron microscope. The results showed： an isolating layer was firstly formed in the cambium zone in the first three days after grafting, then thickened in the next three days and disaggregated after the contents were absorbed by the callus cells, and at last disappeared on the 12th day. A series of healing responses in callus cells were induced during this process. After grafting, the plasmodesmata of the cells near the grafting face were closed while the others away from the surface were still active in transportation. Cell walls of some cells close to the isolating layer were degraded and the protoplasm was fused. The quantity of organelles such as mitochondria, endoplasmic reticulums and plastids was increased. Many multi-vesicular bodies and paramural bodies were observed. Starch granules were accumulated at the beginning and disappeared afterward.

关 键 词： 山核桃 嫁接 愈伤反应 隔离层 超微结构

领　　域： [农业科学]