作　　者： ; ; ;

机构地区： 深圳大学生命科学学院过敏反应与免疫学研究所

出　　处： 《食品科技》 2009年第6期294-296,共3页

摘　　要： 目的:对大闸蟹的主要过敏原进行分析、鉴定与纯化。方法:过SDS-PAGE电泳分离大闸蟹的蛋白质组分,采用免疫印迹(Western-blotting)方法鉴定过敏原,通过离子交换层析对大闸蟹主要过敏原进行初步纯化。结果:大闸蟹粗提液SDS-PAGE显示,其蟹身、四肢和蟹黄的蛋白条带不同,大闸蟹全身含量高的蛋白有9条,Western-Blotting显示蟹全身蛋白出现了5条明显的阳性条带,分子量分别为25、23、21、19 ku和11 ku。离子交换层析可初步纯化出11 ku的过敏原蛋白。结论:对大闸蟹过敏原成功进行分离和鉴定,并初步纯化出腰果的主要过敏原。 Objective： To isolate, identify and purify the allergens in crabs, Eriocheir sinensis H.. Methods： The total protein of the crabs was isolated by SDS-PAGE. The allergens of crabs were identified by Westernblotting. To purify the allergens, ion exchange chromatography was employed. Results： There were different protein bands in crabs different position, there are nine high concertration protein bands visible on the SDSPAGE gel in crabs hole body. The allergens whose molecular mass were 25 ku, 23 ku, 21 ku, 19 ku and 11 ku. The 11 ku allergen of crabs was purified by ion exchange chromatography. Conclusion： The allergens of crabs were isolated and identified. And the allergen of crabs was purified primarily.

关 键 词： 大闸蟹 过敏原 离子交换层析

领　　域： [农业科学] [农业科学]