作　　者： ; ; ; ; ;

机构地区： 仲恺农业工程学院生命科学学院

出　　处： 《中国生物工程杂志》 2009年第6期52-57,共6页

摘　　要： 通过遗传学手段,构建了细极链格孢菌(Alternaria tenuissima)cDNA酵母表达文库并筛选获得MAPK激酶AtPBS2基因,该基因全长2492bp,编码683个氨基酸。AtPbs2p与来源于烟曲霉(Aspergillus fumigatus)的AtPbs2p(XP_752961)、粗糙脉孢菌(Neurospora crassa)的NcPbs2p(XP_965727)、稻瘟菌(Magnaporthe grisea)MGCH7(XP_001522946)及酵母(Saccharomyces cerevisiae)ScPbs2p(EDN63254)序列分别具有52%、52%、49%和47%的相似性。在高渗环境下,AtPBS2基因与酵母的ScPBS2基因功能相同,具有耐高渗透压环境的能力。细极链格孢菌(A.tenuissima)中存在HOG通路信号途径,AtPBS2基因可能与链格孢菌(A.tenuissima)的逆境适应性调节密切相关。 An Alternaria tenuissima cDNA yeast expression library was constructed. The MAPK kinase PBS2 was isolated from an Alternaria tenuissima cDNA expression library, designated as AtPBS2. It has a size of 2492 bases in length, encoded a protein of 683 amino acids. The AtPbs2P amino acid sequence shows 52% ,52%, 49% and 47% identities with AfPbs2P （ XP_752961 ） of Aspergillus fumigatu, MGCH7 （ XP_001522946 ） of Magnaporthe grisea and ScHogl P （EDN63254） of Saccharomyces cerevisiae, respectively. AtPBS2 cDNA sequences could complement the functions of S. cerevisiae ScPBS2 genes in sodium tolerance, suggesting a functional HOG pathway exists in A. tenuissima, AtPBS2 gene was involved in the stress adaptation regulation of A. tenuissima.

关 键 词： 激酶 细极链格孢菌 途径 基因

领　　域： [生物学]