作　　者： ;

机构地区： 韩山师范学院化学系

出　　处： 《安徽农业科学》 2009年第17期7826-7826,7851,共2页

摘　　要： [目的]建立双波长分光光度法同时测定芦荟中钙和镁的方法。[方法]编制计算机程序选择最佳波长对，研究测定钙和镁的试验条件。[结果]结合稳定性因素，选择520～592nm为钙的测定波长对，选择564～610nm为镁的测定波长对。钙和镁的线性回归方程分别为：A=0．00714＋0．01625C、A=0．00384＋0．00987C；样品平均加标回收率钙为96．1％～103．0％，镁为98．2％～103．0％。[结论]该方法重现性和准确性好，可以作为芦荟中钙、镁的测定方法。 [Objective]The aim was to establish the method of dual-wavelength spectrophotometry for the determination of calcium and magnesium in Aloe. [ Method] A computer program is compiled to select the optimum pairs of wavelength, the condition for the determination of calcium and magnesium was studied. [ Result]According to the stability factor, the pairs of wavelength of the determination of calcium and magnesium were selected at 520- 592 nm and 564 - 610 nm, respectively. The linear equation of calcium and magnesium were A = 0.007 14 ＋ 0.016 25 C, A = 0.003 84 ＋ 0.009 87 C, the recovery ranges were 96.1% - 103.0% and 98.2% - 103.0%. [Conclusion]the method has a good accuracy and reproducibility, it can be used as an analysis method for calcium and magnesium in Aloe.

关 键 词： 双波长分光光度法 芦荟 钙 镁

领　　域： [理学] [理学]