作　　者： ;

机构地区： 韩山师范学院生物系

出　　处： 《生物技术通报》 2009年第6期71-74,共4页

摘　　要： 分离并克隆差异表达基因是生命科学的研究热点。近年来,以差示筛选、扣除杂交等基本方法为基础,先后出现了抑制差减杂交,微阵列技术等多种分析差异表达基因的技术,使差异表达基因分离方法不断完善。对这些方法的优缺点、发展趋势及应用前景进行了简要综述。 Cloning differential expressed genes has become prime interest in molecular biology. In recent years, a variety of methods have been developed for analying differentially expressed genes, including differential display reverse transcription-PCR （ DDRT-PCR）, representational difference analysis （ RDA）, suppression subtractive hybridization （ SSH ） and cDNA microarray. This review described the principles, technical routes, improved methods, advantages and defects of these methods. Moreover, Associated applications and prospects in related studies were discussed.

关 键 词： 差异表达基因 差示筛选 扣除杂交 差异显示技术 指纹图谱技术 代表性差异分析 抑制差减杂交 微阵列技术

领　　域： [生物学] [化学工程]