作　　者： ; ; ; ; ;

机构地区： 仲恺农业工程学院生命科学学院

出　　处： 《畜牧兽医学报》 2009年第5期652-657,共6页

摘　　要： 旨在探讨马岗鹅产蛋-就巢周期内卵泡发育的内分泌调控机制。试验1:观察了马岗鹅产蛋-就巢周期内PRL、LH、P4和INB水平的变化和就巢期(第1天)、就巢终止期(第10天)、开产期(第25天)、产蛋高峰期(第40天)、停产期(第55天)和就巢期(第70天)卵泡的发育。试验2:在对马岗鹅终止就巢时(第1天)主动免疫重组鸡PRL蛋白(1 mg.只-1),并在开产前(第23天)和产蛋高峰期(第45天)加强免疫(0.8和0.5 mg.只-1),同时从第33天将每天光照由11 h增至16 h,观察对马岗鹅产蛋和就巢的影响。试验1结果表明,随着就巢的终止,PRL水平下降,LH、P4和INB水平上升。PRL在开产前降至最低,开产后又逐渐上升,就巢期达最高;LH则在开产前和产蛋期呈现两波分泌峰;P4和INB与PRL呈相反变化,在产蛋高峰期最高,就巢期最低。鹅群在第24天恢复产蛋,开产前有约10枚大白卵泡(LWF)发育为小黄卵泡(SYF)和大黄卵泡(LYF);在约30 d的产蛋期内,平均每只产蛋约8枚,产蛋结束后90%的鹅发生就巢。试验2结果表明,对马岗鹅主动免疫PRL蛋白一定程度推迟(P<0.05)了开产后产蛋率的上升,并一定程度抑制就巢的发生;而在延长光照15 d后,免疫组的产蛋和就巢则快速升至与对照组相当水平。整个试验期,2组累计就巢均达到100%,但免疫组比对照组多产蛋1枚(8.0 vs 7.0)。结果提示,PRL和LH的交替分泌调控马岗鹅的产蛋就巢周期;卵泡发育时分泌的INB,以及由P4促进分泌的PRL,调节周期中卵泡发育和产蛋的数量。PRL不仅促进就巢发生并导致周期内较低等级卵泡的闭锁,可能还在开产前后具有促进卵泡发育和产蛋的作用。 This study was conducted to investigate the endocrine regulatory mechanisms of follicular development in the lay-incubation cycle of Magang geese. Experiment 1. investigated the changes in palsma concentrations of PRL, LH, P4, and INB during the cycle, and the follicular development at incubation （d0）, end of incubation （d10）, onset of lay （d25）, peak of lay （d40）, end of lay （d55） and incubation （d70） in the lay-incubation cycle. Experiment 2, after being deprived of laying nest to terminate incubation behaviour on day 1 （d1）, Magang geese were immu- nized intramuscularly on clays 1, 23 and 45, with 1 mL of immunogen containing 1, 0. 8, and 0.5 mg, respectively, of recombinant chicken PRL protein. The photoperiod under which the geese were kept was increased from 11L.13D to 16L：8D on day 33. In expreiment 1, with the termination of incubation, PRL concentration in plasma decreased, whereas the concentrations of LH, P4 and INB increased. PRL concentrations fell to lowest levels prior to the onset of lay and rose to high levels during laying to the peak when incubation initiated. LH concentration in plasma exhibited a bi-phasic pattern in the whole cycle. The patterns of P4 and INB concentrations were opposite to that of PRL, reaching to high levels at peak lay and to the lowest at incubation. Laying resumed on day 24 in the lay-incubation cycle, and approximately 10 follicles were recruited into hierarchical development before onset of lay, among which 8 developed to ovulation and were laid as eggs. Ninety percent of the geese exhibited incubation behabviour after laying one clutch of approximately eight eggs in approximately 30 days. Results of experiment 2 showed that immunization against PRL increased the clutch size by one egg （8.0 vs 7.0）, although initially retarded the rise of laying rate （P〈0.05）. Development of incubation behaviour was also delayed by one week in the PRL immunized geese, which still reached to 100% as in the control geese. Results of the two experi

关 键 词： 马岗鹅 产蛋 就巢周期 卵泡发育 调控

领　　域： [农业科学] [农业科学] [农业科学] [农业科学]