作　　者： ; ; ; ; ;

机构地区： 中国农业科学院北京畜牧兽医研究所

出　　处： 《遗传》 2009年第6期615-619,共5页

摘　　要： 文章以BF基因为产仔数的候选基因,运用PCR-RFLP方法对大白猪BF基因内含子1序列进行分析,发现内含子1第79bp处发生了C→T突变,命名为BF-intron1-C79T。所扩增PCR产物经SmaⅠ酶切后,可分出3种基因型,分别为CC、CT和TT。经χ2适合性检验,大白猪群体在该位点达到了Hardy-Weinberg平衡状态。将不同基因型与总产仔数(TNB)、产活仔数(NBA)、初生重(BW)和胎盘效率(PE)进行关联分析,结果发现,初产母猪在该位点上,CC基因型比CT基因型个体的TNB和NBA均多3.10头,但差异不显著(P>0.05);在BW和PE上,CC基因型均高于CT基因型,差异不显著(P>0.05)。经产母猪在该位点上,CC基因型比TT基因型个体的TNB和NBA分别多3.45头和3.92头,PE高23.80%,均达到显著水平;但CT基因型个体与CC和TT个体之间的TNB、NBA和PE差异不显著(P>0.05)。因此,BF-intron1-C79T位点可作为繁殖性状及胎盘效率的潜在分子育种标记,具有很大的研究价值。 Taking BF gene as one of the candidate genes influencing on reproductive traits in present study, this study used PCR-RFLP analyzised the polymorphism of intron 1 of BF gene in Large White sows. A single nucleotide polymorphism （SNP） named as BF-intron 1-C79T was detected. And the three genotypes of CC, CT,TT got fitted Hardy-Weinberg equilibrium with χ^2-fitness test. An association analysis was tested between the genetic polymorphism at intron 1 and total number born （TNB）, number born alive （NBA）, born weight （BW） and placental efficiency （PE） respectively. It showed that, at first parity, the TNB, NBA, BW and PE with CC genotype were higher than those with CT genotype but not significant （P〉0.05）. Meanwhile, in multiparous sow population, the TNB, NBA and PE with CC genotype were significantly more than those with TT genotype （P〈0.05）, with increase of 3.45, 3.92, and 23.80%, respectively. Thus BF-intron 1-C79T is suggested to be a potential genetic marker for the breeding on the reproductive traits and placental efficiency.

关 键 词： 基因 多态性 繁殖性状 胎盘效率

领　　域： [农业科学] [农业科学] [农业科学] [农业科学]