作　　者： ; ; ;

机构地区： 华南农业大学食品学院

出　　处： 《水产科学》 2009年第5期280-283,共4页

摘　　要： 利用纤维素酶辅助破壁,显著提高了紫菜藻红蛋白的提取率。比较了纤维素酶结合反复冻融破壁、超声波破壁、自溶破壁等各种方法的提取效果,最终确定选用纤维素酶+反复冻溶破壁法提取藻红蛋白。并确定提取条件:添加纤维素酶50μl/g干紫菜,于4℃、pH 7.0条件下作用2 h,反复冻融3次,在此条件下提取率达1.55%,纯度1.36。室温、光照、Fe2+、Zn2+和Cu2+等金属离子存在均会破坏藻红蛋白。 The cellulase was used to lyse laver cell wall whose phycoerythrin was extracted by different methods including repeated freezing-thawing, ultrasonic treatment and autolysis. Good extraction with extraction rate of 1.55% and purity of 1.36 was achieved by the repeated freezing-thawing in combination with cellulase treatment under the following conditions： 1 g laver treated with 50 μl cellulase at 4℃ pH 7.0, repeating the freezing and thawing procedure for 3 times. The laver phycoerythrin was found to be easily destroyed by room temperature, sunlight and presence of metal ions such as Fe^2＋ ,Zn^2＋ and Cu^2＋.

关 键 词： 紫菜 藻红蛋白 纤维素酶 反复冻溶 稳定性

领　　域： [轻工技术与工程] [轻工技术与工程]