作　　者： ;

机构地区： 中山大学生命科学学院生物工程研究中心

出　　处： 《Acta Genetica Sinica》 1998年第1期80-85,共6页

摘　　要： 运用异核体对生物钟基因突变型ｐｒｄ－４和野生型ｐｒｄ－４＋的显隐性关系进行了研究。经多次回交除去１０多个异核体不相容位点的等位基因差异。在回交前分别导入肌醇缺陷型和泛酸缺陷型突变。回交后代分别含有上述两遗传突变，并分别含有ｐｒｄ－４和ｐｒｄ－４＋位点。由于遗传互补，从回交后代构建的异核体能在基本培养基上生长。异核体的ｐｒｄ－４／ｐｒｄ－４＋核比例即为肌醇缺陷型／泛酸缺陷型核比例。分别在２５℃和２９℃确定异核体近似日周期。结果表明ｐｒｄ－４对ｐｒ－４＋呈不完全显性。 Through heterokaryon studies, the dominance-recessiveness relationship of biological clock gene mutant prd -4 and prd -4 + has been determined. During the construction of heterokaryons, the allelic differences of more than 10 heterokaryon incompability loci have been eliminated after 5 backcrosses. inl and pan -2 mutations were initially introduced into the parental strains. The progeny of the backcrosses contain either inl or pan -2 markers, and additionally, prd -4 or prd -4 +. Heterokaryons constructed from the the progeny of the final cross are able to grow in minimal medium because of genetic complementation. The nuclear ratio of prd -4/ prd -4 + in the heterokaryons is derived from nuclear ratio of inl/pan . The period length of heterokaryons is determined at both 25℃ and 29℃. The data indicate that prd -4 exhibits incomplete dominance over pre -4 +.

关 键 词： 异核体 显隐性 生物钟 突变型 野生型

领　　域： [生物学]