作　　者： ; ; ; ; ;

机构地区： 华中农业大学食品科学技术学院

出　　处： 《中国酿造》 2009年第5期73-76,共4页

摘　　要： 利用分子印迹技术,制备用于4-甲基咪唑(4-MI)快速检测的高分子材料。以4-甲基咪唑为模板分子,α-甲基丙烯酸(MAA)为功能单体,偶氮二异丁腈(AIBN)为引发剂,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,在乙腈中通过沉淀聚合制备了分子印迹聚合物。用紫外分光光度法对4-MI与MAA的相互作用进行了分析,结果表明主客体主要存在形式为,1个4-MI为1个MAA所包围。利用Langmuir数学模型对吸附特性进行了分析,Scatchard图显示印迹聚合物的最大吸附量Bmax=221.14μmol/g和解吸常数KD=1.8mmol/L。同时对印迹聚合物的吸附选择性和吸附动力学进行了初步研究。 The polymer used for quick determination of 4-methyl imidazole （4-MI） was prepared by molecular imprinting. The uniformly imprinted polymer microspheres were prepared by precipitation polymerization in acetonitrile, using 4-MI as template, methacrylic acid （MAA） as functional monomer, azobisisobutyronitrile （AIBN） as initiator and ethylene glycol dimethacrylate （EGDMA） as cross-linker. The UV spectrophotometry was used to demonstrate the interaction between 4-MI and MAA. It was found that one 4-MI was entrapped by one MAA molecules, which was the main existing form of subject and object. Langmuir model was used to analyze the adsorption data. The Scatchard chart showed the apparent maximum binding capacity （Bmax） and the dissociation contents （KD） of the imprinted polymer were 221.14 μmol/g and 1.8 mmol/L, respectively. The absorption selectivity and absorption kinetics of imprinted polymer were preliminarily studied.

关 键 词： 分子印迹技术 甲基咪唑 沉淀聚合

领　　域： [生物学]