作　　者： ; ; ; ;

机构地区： 南方医科大学南方医院

出　　处： 《功能材料》 2009年第5期836-839,共4页

摘　　要： 应用氧化葡聚糖与精胺通过还原胺法反应,制备了聚阳离子非病毒基因载体葡聚糖-寡胺化合物;用红外光谱测定仪、核磁共振波谱仪、紫外光谱仪,激光纳米粒度测定仪、透射电子显微镜进行了表征。证实了该载体为葡聚糖-精胺化合物DSP。其平均粒径为170nm,Zeta电位为+31.1mV,镜下呈园球形状。将载体DSP与pCMV-GFP的质粒DNA以8∶1(N/P)组成复合物时,对SMMC-7721人肝癌细胞进行体外转染,转染效率高于阳性对照组transfectine 2000,为31.1%。实验表明该材料有望成为临床的非病毒基因载体。 The dextran-spermine polycations （DSP） as a non-virus gene vector have been prepared by the reduced-amine reaction between oxided dextran and spermine. This structure of compounds has been proved by IR,^1H-NMR spectroscopy. The particles size and zeta potential were 170nm and ＋31. lmV respectively in the average,measured by malvern zetasizer 3000HS instrument. The polyplexes were formed by the p CMV-GFP plasmid DNA combined with the vector when the N/P ratio of the carrier to DNA is 8 to 1. The highest yield of tranfection efficiency were found to be about 31.1% ,as higher than the transfectine 2000 when the the polyplexes were transfected to SMMC-7721 cell at the N/P ratio of 8：1. This results indicates this dextran-spermine polycation is possible to become a new gene vector in the future clinic application.

关 键 词： 葡聚糖 精胺 聚阳离子 复合物 转染率

领　　域： [生物学]