机构地区: 甘肃农业大学
出 处: 《江西农业学报》 2009年第4期101-103,共3页
摘 要: 从T载体上得到的目的基因和dhfr共扩增基因真核表达载体构建了pCI-PrP102。纯化后的重组质粒通过脂质体转染CHO/dhfr-细胞,经MTX加压筛选获得稳定表达的细胞株,间接免疫荧光试验检测到目的蛋白的表达。 :The target genes from T - vector were connected with dhfr co - amplified gene of eukaryotic vector and constructed pCI -PrP102. Purified recombinant plasmids were transferred into CHO/dhfr- cell through liposome, and obtained the stable expression of the cell strains after MTX screening, and indirect immunity fluorescence analysis detected the expression of the goal protein.
关 键 词: 牛朊蛋白 二氢叶酸还原酶缺陷型 细胞 稳定表达